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ETILTEST Saliva alcohol test The Ethyl test is an enzymatic test for the rapid and safe detection of alcohol (ALC) (ethyl alcohol/ethanol) in saliva and other fluid samples. By analyzing the saliva sample, it is possible to determine the blood alcohol concentration. This enzymatic analysis produces a qualitative result, meaning that the test shows whether or not alcohol is present in the samples. The test detects an ethanol concentration of 0.1 or 10 mg/dl, which is visualized by a light green-gray color on the reagent pad or test stick. The green color becomes more intense as the alcohol concentration in the sample increases. Therefore, in the presence of alcohol in the sample, the ethyl test causes a color change from light green-gray at an alcohol concentration of 0.1 mg/dl, to a slightly deeper green at an intermediate concentration, and finally to a dark green-gray color as the alcohol concentration approaches 1.5 mg/dl. We recommend using this visual test only as a qualitative test, as the discriminatory capacity of the human eye can cause errors in quantitative results. Measures 5 levels of alcohol in the body: 0.00 g/l - 0.20 g/l - 0.50 g/l - 0.80 g/l - 1.50 g/l. The alcohol test consists of a plastic stick with a reagent pad on the end. When the pad comes into contact with alcohol solutions, it quickly turns green or blue, depending on the amount of alcohol in the liquid. The reagent pad reacts based on a solid-phase chemical synthesis. How to use 1. Do not put anything in your mouth for fifteen (15) minutes before the test begins. This includes soft drinks, tobacco products, coffee, mints, food, etc. 2. Bring the sealed bag to room temperature (15-30°C) to avoid moisture condensation on the reagent pad. 3. If testing liquid samples other than saliva, ensure that the liquids are at room temperature before testing. 4. Open the package and remove the test. Examine the test swab at the end of the swab. It should be a light cream color. Any test with a dark brown or stained swab should be discarded. 5. Soak the test pad with liquid or saliva collected from your mouth or a spittoon. Start the timer immediately. 6. After two (2) minutes, observe the color change (if any) on the test pad. If the pad turns green or dark green-gray, the test has detected the presence of alcohol and the result is positive. Results obtained after more than 3 minutes may be incorrect. 7. Estimate the approximate blood alcohol concentration by comparing the color of the test pad with the color scale on the test packaging. We recommend using the visual test as a qualitative test only. Interpretation of results After 2 minutes, read the color of the reagent pad. Do not compare the test result with other color scales. Use only the color scale indicated on the packaging. The reaction occurs more slowly in saliva than in other aqueous solutions. limitation If you do not wait at least 15 minutes after putting food, drink, or other things in your mouth before taking the test, the results may be incorrect due to possible contamination of saliva with interfering substances. The Ethyl test has been designed and calibrated to be interpreted 2 minutes after the reagent buffer has been soaked in. If you wait longer than 2 minutes, the displayed results may be inaccurate or you may get false positive results. The breathalyzer can be used to detect the presence of alcohol in fluids other than saliva. However, when used in this way, the color scale on the package cannot be used as a guide. If alcohol is present in the fluid, the swab will change color from light green-gray to black or cocoa brown as the alcohol concentration increases. If the swab is immersed in pure alcohol, it will stain little or not at all due to the absence of water, which is necessary for the staining reaction to occur. When analyzing drinks, the result can only be considered positive if the swab turns very dark brown. The ethanol test is very sensitive to alcohol. Alcohol vapor, often present in the air, is sometimes detected by the ethanol test. Alcohol vapors are often present in many public places and inside homes. Alcohol is found in many household cleaning products, such as disinfectants, air fresheners, and window cleaners. If alcohol vapors are suspected, testing should be performed in an area where these vapors are not present (e.g., outdoors). Note: The person reading the test results cannot be color blind. controller It is possible to qualitatively verify the integrity of the ethyl test using a solution prepared by adding 4 drops of distilled water with 40% alcohol by volume to a glass of water. With this solution, the tint should match the glass by 0.04% or more (darker). The color reaction with alcohol in saliva occurs more slowly and with a less intense coloration than with alcohol in aqueous solutions. Features Specificity: The Ethyl Alcohol Test will react in the presence of methyl, ethyl and allyl alcohols. The Ethyl Alcohol Test will not react with alcohols containing 5 or more carbon atoms, nor with glycine, glycerol or serine. This property derives from the specificity of the alcohol oxidase enzyme extracted from yeast. Interactions The following substances may interfere with the Ethyl test if liquid samples other than saliva are used: Color enhancing agents: - Peroxide. - Strong oxidants. - (common in detergents, strippers and bleaches). Staining inhibiting agents: Reducing agents: - Ascorbic acid. - Tannic acid. - Pyrogallol. - Mercaptans and tosylates. - Oxalic acid. - Uric acid. - Bilirubin. - L-dopamine. - L-methyldopamine. - Metampyrone. These substances are not usually present in saliva in sufficient quantities to interfere with the test. However, they should not be placed in the mouth for 15 minutes before the test. warnings - Disposable. - Product not intended for medical or diagnostic purposes. - Do not use after the expiration date. - Allow the reagent pad to absorb enough liquid to completely soak it. - Do not eat, drink or smoke while handling samples or the kit. - Do not use the test if the pouch is damaged. - Bring reagents to room temperature (15-30°C) before use. - Do not touch the test plate on the stick to avoid contaminating it. - Avoid contamination of samples with each other by using a new collection container for each sample. - Not to be used for medical or diagnostic purposes. - Handle all specimens as if they contained infectious agents. Observe microbiological precautions during testing and follow standard procedures for proper disposal of specimens. - Leave the stick in the sealed bag until ready to use. - Observe the test result after 2-3 minutes. - Humidity and high temperatures can affect test results. - Do not perform the test in an atmosphere containing alcohol. - Pure alcohol can give false results. - Be aware of possible cross-reactivities. - We recommend using the visual test only as a qualitative test. The person reading the results cannot be color blind. Conservation The test kit should be stored refrigerated or at room temperature (2-30°C) in the sealed pouch, depending on the products shelf life. Bring the stick to room temperature to avoid moisture condensation on the reagent pad. Composition/Ingredient information Complete composition of the kit: - Test strip: contains a filter foil with alcohol oxidase, peroxidase, 3,3,5,5-tetramethylbenzedine (TMB) and dried TRIS-HCl buffer solution. The test strip consists of: PE catalyst, filter paper reagent pad. - Other components: aluminum bag, hygroscopic silica gel. Harmful components: Nobody. Format Pack of 1 piece. Code CALC1S
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