ALCOHOL TEST MEDICAL ASSISTANCE Description Rapid, one-step screening test for the semi-quantitative detection of alcohol in human saliva. Alcohol testing is a rapid and highly sensitive method for detecting the presence of alcohol in human saliva and providing a rough estimate of the relative blood alcohol concentration. This test only provides a preliminary assessment. To obtain a confirmed analytical result, it is necessary to use an alternative, more specific chemical method. How to use Allow the test cassette, specimen, and/or controls to reach room temperature (15-30°C) before testing. Do not put anything in your mouth for 15 minutes before the test begins. This includes soft drinks, tobacco products, coffee, mints and food, etc. Bring the pouch to room temperature before opening. Remove the test from the sealed pouch and use within one hour. Remove the collector from the sealed pouch and insert the sponge end of the collector into your mouth. Vigorously wipe the inside of your mouth and tongue to collect saliva for 3 minutes, until the sponge is completely saturated. Gently pressing the sponge between your tongue and teeth will help saturate it. You should not feel any hard spots on the sponge when it is saturated. Remove the collector from your mouth. Insert the saturated saliva collector into the collection tube and press the sponge fully onto the filter to collect the saliva. Discard the collector. Close the collection tube cap. Place the test cassette on a clean, flat surface. Unscrew the cap of the collection tube. Invert the collection tube, transfer 3 drops of saliva (approximately 120 mcL) into the sample well of the test cassette and start the timer. Avoid trapping air bubbles in the sample well. Replace the screw cap on the collection tube. Read results in 3-5 minutes. Compare the color of the reaction pad to the standard color card to determine the relative blood alcohol concentration. Interpretation of results Positive: The breathalyzer test will produce a color change in the presence of salivary alcohol. The color will range from light blue at a relative blood alcohol concentration of 0.20 g/l to dark blue near a relative blood alcohol concentration of 1.50 g/l. Colored pads are provided in this range to allow an approximation of the relative blood alcohol concentration. The test may produce colors that appear to be present between adjacent colored pads. Negative: If the alcohol test shows no color change, this should be interpreted as a negative result, indicating that no alcohol was detected. Invalid: If the colored pad is blue before applying the saliva sample, do not use the test. Note: The breathalyzer test is very sensitive to the presence of alcohol. A lighter blue color at 0.20 g/l of the colored swab should be interpreted as positive for the presence of alcohol in saliva, but less than 0.20 g/l of relative alcohol in blood. warnings Before performing the test, please read the entire leaflet carefully. For self-testing, not for in vitro diagnostic use. For external use only. Do not use the test after the expiration date printed on the package. Do not use the test if the foil pouch is torn or damaged. Disposable, throw away after first use. The test device should remain in the sealed packaging until use. A contaminated sample may give false results. Keep out of reach of children. Conservation Store at a temperature between 2 and 30°C, in its packaging. Format Pack of 2 pieces. Code MM-DAL-802H